详细说明
Species Reactivity
Human
Specificity
Detects human PS-1 NTF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) PS-1 CTF (aa 298-407) and rhPS-2 NTF (aa 1-80) is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Presenilin-1
Lys1-Lys80
Accession # P49768Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
0.1 µg/mL
Recombinant Human Presenilin‑1 N-Terminal Fragment
Immunohistochemistry
5-15 µg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry | Presenilin‑1 in Human Brain. Presenilin‑1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using 15 µg/mL Goat Anti-Human Presenilin‑1 N‑Terminal Fragment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF149) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ) and counterstained with hematoxylin (blue). View our protocol for . |
Preparation and Storage
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Presenilin-1
Presenilin-1 (PS-1) and Presenilin-2 (PS-2) are integral membrane proteins that contain multiple transmembrane domains. Both are predominantly located within the endoplasmic reticulum (ER) and early Golgi apparatus. They are primarily expressed in neurons and are ubiquitously expressed in the brain. PS-1 and PS-2 are proteolytically cleaved to generate an N-terminal fragment (NTF) and a C-terminal fragment (CTF). Mutations in the gene for Presenilin may play an important role in the pathology of Alzheimer's disease.
Entrez Gene IDs:
5663 (Human)
Alternate Names:
AD3EC 3.4.23.-; Alzheimer disease 3; EC 3.4.23; FAD; presenilin 1; Presenilin1; Presenilin-1; Protein S182; PS-1; PS1presenilin-1; PSEN1; PSNL1; S182