概述

• 产品名称Anti-p53 (phospho T377)抗体[EPR5678]
     

• 描述

  兔单克隆抗体[EPR5678] to p53 (phospho T377)

• 特异性ab133451 only detects p53 phosphorylated at Threonine 377.

• 经测试应用WB

• 种属反应性

  与反应: Human    
   

• 免疫原

  Synthetic phosphopeptide corresponding to a region surrounding Threonine 377 of Human p53 (UniProt P04637).

• 阳性对照    

• Lysate of 293T cells treated with okadaic Acid and calyculin A.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  A trial size is available to purchase for this antibody.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR5678

• 同种型IgG

• 研究领域

• Cancer

• Oncoproteins/suppressors

• Tumor suppressors

• p53 pathway

• Cancer

• Cell cycle

• Cell cycle inhibitors

• p53 pathway

• Epigenetics and Nuclear Signaling

• Cell cycle

• Cell Cycle Inhibitors

• p53

• Epigenetics and Nuclear Signaling

• Transcription

• Cancer susceptibility

• Tumor Suppressors

• Epigenetics and Nuclear Signaling

• DNA / RNA

• DNA Damage & Repair

• DNA Damage Response

• p53

• Cell Biology

• Apoptosis

• Intracellular

• p53 Pathway

• <a javascript:void(0)>Cell Biology

• Cell Cycle

• Cell Cycle Inhibitors

• p53

Anti-p53 (phospho T377) antibody [EPR5678] 图像

• 

  Dot Blot - Anti-p53 (phospho T377) antibody [EPR5678] (ab133451)          

     

  Primary antibody dilution: 1/1000

  Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

  Secondary antibody dilution: 1/2500

  Blocking & dilution buffer: 5% NFDM/TBST

  Lane 1 sample: p53 (pT377) phospho peptide

  Lane 2 sample: p53 non-phospho peptide

  Exposure time: 3 minutes

   

• 

  Western blot - Anti-p53 (phospho T377) antibody [EPR5678] (ab133451)          

     All lanes : Anti-p53 (phospho T377) antibody [EPR5678] (ab133451) at 1/20000 dilution
  
  Lane 1 : HEK293 (human embryonic kidney) treated with Okadaic acid and Calyculin A whole cell lysates
  Lane 2 : Untreated HEK293 (human embryonic kidney) whole cell lysates
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
  
  Predicted band size : 43 kDa
  Observed band size : 53 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 3 minutes    

  Blocking and dilution buffer: 5% NFDM /TBST

   

• 

  Western blot - Anti-p53 (phospho T377) antibody [EPR5678] (ab133451)          

     All lanes : Anti-p53 (phospho T377) antibody [EPR5678] (ab133451) at 1/10000 dilution
  
  Lane 1 : Lysate of 293T cells, untreated
  Lane 2 : Lysate of 293T cells treated with okadaic acid and calyculin A.
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP conjugated goat ant-rabbit antibody at 1/2000 dilution
  
  Predicted band size : 43 kDa
  Observed band size : 53 kDa (why is the actual band size different from the predicted?)