详细说明
概述
产品名称Anti-PPP2CB抗体[EPR11786(B)]
描述
兔单克隆抗体[EPR11786(B)] to PPP2CB
经测试应用IP,ICC/IF,Flow Cyt,WB
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide corresponding to Human PPP2CB.
Database link: P62714阳性对照
293T, HeLa, Human fetal brain, Human fetal heart and NIH 3T3 lysates; 293T and NIH3T3 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR11786(B)
同种型IgG
研究领域
Signal Transduction
Protein Phosphorylation
Ser / Thr Phosphatases
Anti-PPP2CB antibody [EPR11786(B)] 图像
![Immunoprecipitation - Anti-PPP2CB [EPR11786(B)] antibody (ab168371)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab16/ab16837171d.jpg)
Immunoprecipitation - Anti-PPP2CB [EPR11786(B)] antibody (ab168371)
Detection of PPP2CB by Western Blot of Immunprecipitate.
293T cell lysate immunoprecipitated using ab168371 at 1/10 dilution.
For WB detection ab168371 at 1/10000 dilution was used.
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.![Flow Cytometry - Anti-PPP2CB [EPR11786(B)] antibody (ab168371)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab16/ab16837171c.jpg)
Flow Cytometry - Anti-PPP2CB [EPR11786(B)] antibody (ab168371)
Flow Cytometric analysis of permeabilized NIH 3T3 cells labeling PPP2CB with ab168371 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
![Immunocytochemistry/ Immunofluorescence - Anti-PPP2CB [EPR11786(B)] antibody (ab168371)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab16/ab16837171b.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PPP2CB [EPR11786(B)] antibody (ab168371)
Immunofluorescent analysis of 293T cells labeling PPP2CB with ab168371 at 1/50 dilution.
![Western blot - Anti-PPP2CB [EPR11786(B)] antibody (ab168371)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab16/ab16837171a.jpg)
Western blot - Anti-PPP2CB [EPR11786(B)] antibody (ab168371)
All lanes : Anti-PPP2CB antibody [EPR11786(B)] (ab168371) at 1/10000 dilution
Lane 1 : 293T lysate
Lane 2 : HeLa lysate
Lane 3 : Human fetal brain lysate
Lane 4 : Human fetal heart lysate
Lane 5 : NIH3T3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 36 kDa
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