详细说明
概述
产品名称Anti-p38抗体[EPR16878]
描述
兔单克隆抗体[EPR16878] to p38
经测试应用WB,ICC/IF
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human p38 aa 150-250. The exact sequence is proprietary.
Database link: Q16539阳性对照
WB: HeLa, Jurkat, Neuro-2a cell lysates; Human fetal heart, fetal kidney and fetal spleen lysates; Mouse heart, kidney and spleen lysates; Rat heart, kidney and spleen lysates; C6, RAW 264.7, PC12 and NIH 3T3 cell lysates. ICC/IF: HeLa and Jurkat cells. IP: Jurkat cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR16878
同种型IgG
研究领域
Immunology
Innate Immunity
TLR Signaling
Signal Transduction
Protein Phosphorylation
Ser / Thr Kinases
MAPK Pathway
Anti-p38 antibody [EPR16878] 图像
![Western blot - Anti-p38 antibody [EPR16878] (ab182453)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab182453OWB.jpg)
Western blot - Anti-p38 antibody [EPR16878] (ab182453)
Predicted band size : 41 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: p38 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab182453 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab182453 was shown to specifically react with p38 when p38 knockout samples were used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab182453 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.![Western blot - Anti-p38 antibody [EPR16878] (ab182453)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab182453b-1.jpg)
Western blot - Anti-p38 antibody [EPR16878] (ab182453)
All lanes : Anti-p38 antibody [EPR16878] (ab182453) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size : 41 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes5% NFDM/TBST: Blocking and diluting buffer.
![Western blot - Anti-p38 antibody [EPR16878] (ab182453)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab182453b-2.jpg)
Western blot - Anti-p38 antibody [EPR16878] (ab182453)
All lanes : Anti-p38 antibody [EPR16878] (ab182453) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 41 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes5% NFDM/TBST: Blocking and diluting buffer.
![Western blot - Anti-p38 antibody [EPR16878] (ab182453)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab182453b-3.jpg)
Western blot - Anti-p38 antibody [EPR16878] (ab182453)
All lanes : Anti-p38 antibody [EPR16878] (ab182453) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat kidney lysate
Lane 6 : Rat spleen lysate
Lane 7 : C6 (Rat glial tumor cells) whole cell lysate
Lane 8 : RAW 264.7(Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 9 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 10 : NIH 3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size : 41 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds5% NFDM/TBST: Blocking and diluting buffer.
![Immunocytochemistry/ Immunofluorescence - Anti-p38 antibody [EPR16878] (ab182453)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab182453f-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-p38 antibody [EPR16878] (ab182453)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling p38 with ab182453 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing cytoplasm and nucleus staining on HeLa cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
1. ab182453 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.![Immunocytochemistry/ Immunofluorescence - Anti-p38 antibody [EPR16878] (ab182453)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab182453f-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-p38 antibody [EPR16878] (ab182453)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling p38 with ab182453 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing cytoplasm and nucleus staining on Jurkat cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
1. ab182453 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.![Immunoprecipitation - Anti-p38 antibody [EPR16878] (ab182453)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab182453-ip.jpg)
Immunoprecipitation - Anti-p38 antibody [EPR16878] (ab182453)
Immunoprecipitation of p38 from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate achieved using ab182453 at 1/100 dilution.
Lane 1: Input: 10µg of Jurkat whole cell lysate.
Lane 2: Jurkat whole cell lysate following IP with ab182453.
Lane 3: negative control: IP using Rabbit monoclonal IgG (ab172730) instead of ab182453 in Jurkat whole cell lysate.
Western blot was performed using ab182453 at 1/1000 dilution.
An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. 10 second exposure.
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