概述

• 产品名称Anti-p95 NBS1 (phospho S343)抗体[EP178]
     

• 描述

  兔单克隆抗体[EP178] to p95 NBS1 (phospho S343)

• 特异性ab109453 only detects p95 NBS1 phosphorylated on Serine 343.

• 经测试应用WB,IP,ICC

• 种属反应性

  与反应: Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human p95 NBS1 (phospho S343). The exact sequence is proprietary.

• 阳性对照    

• WB: Jurkat cell lysates, treated with Etopside.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

   

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EP178

• 同种型IgG

• 研究领域

• Cancer

• Oncoproteins/suppressors

• Tumor suppressors

• Other

• Epigenetics and Nuclear Signaling

• DNA / RNA

• DNA Damage & Repair

• Homologous Recomb.

• <a javascript:void(0)>Epigenetics and Nuclear Signaling

• DNA / RNA

• DNA Damage & Repair

• Non Homol. End Joining

Anti-p95 NBS1 (phospho S343) antibody [EP178] 图像

• 

  Dot Blot - Anti-p95 NBS1 (phospho S343) antibody [EP178] (ab109453)          

     

  Dot blot analysis of p95 NBS1 (pS343) peptide (Lane 1) and p95 NBS1 non-phospho peptide (Lane 2) labelling p95 NBS1 (phospho S343) with ab109453 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

  Blocking and dilution buffer: 5% NFDM/TBST.

  Exposure time: 3 minutes.

   

• 

  Western blot - p95 NBS1 (phospho S343) antibody [EP178] (ab109453)          

     All lanes : Anti-p95 NBS1 (phospho S343) antibody [EP178] (ab109453) at 1/100 dilution
  
  Lane 1 : Jurkat cell lysate, untreated
  Lane 2 : Jurkat cell lysate, treated with Etopside
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 84 kDa
  Observed band size : 95 kDa (why is the actual band size different from the predicted?)