详细说明
Species Reactivity
Human
Specificity
Recognizes the human NKG2A/CD94 heterodimer. It does not recognize the NKG2C/CD94 heterodimer or the CD94 homodimer.
Source
Monoclonal Mouse IgG 2A Clone # 131411
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2A and CD94
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Biotin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of NKG2A/CD159a in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # ) and either (A) Mouse Anti-Human NKG2A/CD159a Biotinylated Monoclonal Antibody (Catalog # FAB1059B) or (B) Mouse IgG2A Biotinylated Isotype Control (Catalog # ) followed by Streptavidin-Phycoerythrin (Catalog # ). |
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: NKG2A/CD159a
NKG2A is a type II transmembrane receptor having a single extracellular lectin-like domain and a cytoplasmic ITIM motif. It associates with CD94 and is expressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its ligand, HLA-E.
Long Name:
Natural Killer G2A
Entrez Gene IDs:
3821 (Human); 16641 (Mouse)
Alternate Names:
CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B