• Species Reactivity

  Mouse

• Specificity

  Detects mouse IL-34 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-34 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 780310

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse IL-34    
  Asn21-Pro235    
  Accession # Q8R1R4

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.01 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Neutralization

  Measured by its ability to neutralize IL‑34-induced proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line. The Neutralization Dose (ND    ₅₀) is typically 0.1-0.5 ug/mL in the presence of 100 ng/mL Recombinant Mouse IL‑34.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-34

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77 homolog) is a 39 kDa, secreted cytokine that belongs to no known cytokine family (1). Mouse IL-34 is synthesized as a 235 amino acid (aa) precursor with a 20 aa signal sequence and a 215 aa mature chain that contains two potential sites for N-linked glycosylation. There are three isoforms for IL-34. Isoform 1 (Q8R1R4-1) is the recombinant mouse IL-34 described in this insert. Isoform 2 (Q8R1R4-2) has an 85 aa substitution for the final 101 aa in isoform 1, and isoform 3 (Q8R1R4-3) lacks Q81 in isoform 1. Mouse IL-34 shares 71% aa sequence identity with human IL‑34. IL‑34 is expressed in various tissues, including heart, brain, lung, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and it is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF‑1R) (1). IL-34 stimulates monocyte viability (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).

• References:

1. Lin, H. et al. (2008) Science 320:807.

• Long Name:

  Interleukin 34

• Entrez Gene IDs:

  146433 (Human); 76527 (Mouse)

• Alternate Names:

  C16orf77; IL34; IL-34