• Species Reactivity

  Mouse

• Specificity

  Detects mouse Cystatin B in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Cystatin B is observed and no cross-reactivity with rhCystatin A, C, D, E/M, F, S, SA, rhFetuin A, rhFetuin B, rhHPRG, or rhKininogen is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 227807

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse Cystatin B    
  Met2-Phe98    
  Accession # Q62426

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  Perfusion fixed frozen sections of mouse spinal cord

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin B

Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Cystatin B-deficient mice have increased expression of proteolysis, apoptosis and glial activation genes, which is consistent with the pathology found in the mouse model of human progressive myoclonus epilepsy (EPM1) (3). The mouse Cystatin B consists of 98 amino acid residues (4).

• References:

1. Abrahamson, M. (1994) Methods Enzymol. 244:685.

2. Pol, E. and I. Bjork (1999) Biochemistry 38:10519.

3. Lieuallen, K. et al. (2001) Hum. Mol. Genet. 10:1867.

4. Pennacchio, L.A. and R.M. Myers (1996) Genome Res. 6:1103.

• Entrez Gene IDs:

  1476 (Human); 13014 (Mouse)

• Alternate Names:

  CPI-B; CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B; CSTB; cystatin B (stefin B); Cystatin B; EPM1; Liver thiol proteinase inhibitor; PME; Stefin B; stefin-B