• Species Reactivity

  Human

• Specificity

  Detects human SLITRK1 in direct ELISAs. In direct ELISAs, no cross-reactivity with reactivity with SLITRK2, 3, 4, or 5 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 327829

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human SLITRK1    
  Ala16-Ser616    
  Accession # Q96PX8

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  Immersion fixed paraffin-embedded sections of human brain (cortex)

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLITRK1

SLITRK1 (Slit and Trk-like family member 1) is a charter member of the SLITRK family of proteins (1, 2). This family includes six members, all of which contain a slit‑like extracellular region, and all of which (save for SLITRK1) contain a Trk‑like cytoplasmic region (3). Human SLITRK1 is a type I transmembrane protein 696 amino acids (aa) in length. It contains a 17 aa signal sequence, a 605 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 53 aa cytoplasmic region. The ECD is characterized by the presence of two leucine-rich domains (LRD) that resemble those found in slit. Each LRD (aa 57‑200; aa 374‑517) contains six leucine-rich repeats (LRRs), followed by a Cys-rich, C-terminal LRR (aa 212‑262; aa 529‑579). The exact function of the LRRs is not known. Based on other LRRs, it might be assumed that they mediate protein-protein-interaction (4). There are multiple, single aa mutations in the ECD. Their significance is unclear (5). One truncating frameshift mutation is known that shows a unique 27 aa substitution after Arg 421 (6). This 448 aa form is associated with Tourette’s Syndrome. As noted, the cytoplasmic region is unique among SLITRK family members. It contains no known signaling motifs, which is in contrast to SLITRK2‑6 which show TrkA-like tyrosine phosphorylation sites. This difference is reflected in their function. SLITRK1 is a protein that is found in adult neurons of the cerebrum, thalamus and hippocampus. It induces neurite outgrowth whereas SLITRK2‑6 block neurite extension (3). Human SLITRK1 shares 99%, 98% and 99% aa sequence identity with canine, mouse and bovine SLITRK1, respectively.

• References:

1. Aruga, J. et al. (2003) Gene 315:87.

2. Nagase, T. et al. (2001) DNA Res. 8:179.

3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.

4. Enkhbayar, P. et al. (2003) Proteins 54:394.

5. Zuchner, S. et al. (2006) Mol. Psychiat. 11:888.

6. Abelson, J.F. et al. (2005) Science 310:317.

• Long Name:

  SLIT and NTRK-like Family, Member 1

• Entrez Gene IDs:

  114798 (Human); 76965 (Mouse)

• Alternate Names:

  FLJ54428; KIAA1910KIAA0918; leucine rich repeat containing 12; Leucine-rich repeat-containing protein 12; LRRC12; RP11-395N17.1; SLIT and NTRK-like family, member 1; SLIT and NTRK-like protein 1; slit and trk like gene 1; SLITRK1; TTM