• Species Reactivity

  Human, Mouse

• Specificity

  Detects mouse Wnt-3a in direct ELISAs.    
   

• Source

  Recombinant Monoclonal Rat IgG    _2A Clone # 217804.2R

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  E.coli-derived recombinant mouse Wnt-3a    
  Ser36-Gln75, Trp219-Arg269    
  Accession # P27487

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Intracellular Staining by Flow Cytometry

  0.25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-3a

Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1‑3). Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase (1, 4). Mouse Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity (6, 7). A second site at Ser 209 is modified by palmitoleic acid and also contributes to activity and secretion (8). Mouse Wnt-3a shares 96% amino acid (aa) identity with human Wnt-3a, and 97% with bovine and canine Wnt-3a. The rat Wnt-3a precursor as it is apparently translated shares 100% aa identity with mouse Wnt-3a aa 63-352 (9). Wnt-3a also shares 87% aa identity with Wnt-3. During development, Wnt-3a is morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination, and is expressed at the same locations and times as Wnt-2b and Wnt-5a (10). When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation (10-13). Recombinant Wnt-3a promotes proliferation of committed stem cell lineages in vitro, and may help maintain the cells in an undifferentiated state (6, 14) For example, Wnt-3a can induce self-renewal of hematopoietic stem cells, allowing expansion without further differentiation (6).

• References:

1. Mikels, A.J. and R. Nusse (2006) Oncogene 25:7461.

2. Miller, J.R. (2001) Genome Biol. 3:3001.

3. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.

4. Lu, W et al. (2004) Cell 119:97.

5. Smolich, B.D. et al. (1993) Mol. Biol Cell 4:1267.

6. Willert, K. et al. (2003) Nature 423:448.

7. Komekado, H. et al. (2007) Genes Cells 12:521.

8. Takada, R. et al. (2006) Dev. Cell 11:791.

9. Entrez Accession # NP_001100475.

10. Dunty, W.C. et al. (2008) Development 135:85.

11. Lee, S.M.K. et al. (2000) Development 127:457.

12. Takada, S. et al. (1994) Genes Dev. 8:174.

13. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.

14. Nusse, R. (2008) Cell Res. 18:523.

• Long Name:

  Wingless-type MMTV Integration Site Family, Member 3a

• Entrez Gene IDs:

  89780 (Human); 22416 (Mouse)

• Alternate Names:

  MGC119418; MGC119419; MGC119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt3a; Wnt-3a