• Species Reactivity

  Human

• Specificity

  Detects human DLL3 in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) DLL1 or rhDLL4.

• Source

  Monoclonal Mouse IgG    _2B Clone # 378703

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human DLL3    
  Ala27-Pro488    
  Accession # Q9NYJ7

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human DLL3

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL3

Delta-like 3 (DLL3) is a transmembrane Delta-like protein that contains one DSL domain and six EGF-like repeats. DLL3 is critical for somite segmentation and neurogenesis during early embryonic development. It does not bind Notch receptors but functions as an antagonist of Notch signaling. Defects in DLL3 cause axial skeletal abnormalities in spondylocostal dysostosis. Within the extracellular domain, human DLL3 shares 84% amino acid sequence identity with mouse and rat DLL3.

• Long Name:

  Delta-like 3

• Entrez Gene IDs:

  10683 (Human); 13389 (Mouse); 114125 (Rat)

• Alternate Names:

  delta (Drosophila)-like 3; Delta3; delta-like 3 (Drosophila); delta-like protein 3; DLL3; Drosophila Delta homolog 3; Pudgy; SCDO1; SCDO1delta3