• Species Reactivity

  Mouse

• Specificity

  Detects mouse MAdCAM-1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) MAdCAM-1, recombinant mouse (rm) ICAM-1, rmICAM-2, rmICAM-5, rmVCAM-1, and rhICAM-3.    
   

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse MAdCAM-1    
  Gln22-Thr365, Predicted    
  Accession # NP_038619

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.25 µg/mL

  See below

• 
  

• Immunohistochemistry

  5-15 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAdCAM-1

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an immunoglobulin (Ig) cell adhesion molecule family member. In addition to Ig domains, it contains a mucin-like domain and a membrane proximal domain with similarity to IgA. MAdCAM-1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyer’s patches. It has also been found to be expressed on sinus-lining cells of the spleen. The integrin, alpha ₄ beta ₇, has been shown to function as the MAdCAM-1 receptor. The Ig domains of MAdCAM-1 have been found to be critical to alpha ₄ beta ₇ binding. The mucin domain has been shown to have activity in L-Selectin binding. MAdCAM-1 expression has been demonstrated to be up-regulated by TNF-alpha and IL-1 beta. MAdCAM-1 appears to play a role in inflammatory bowel disease (IBD) as its expression is highly up-regulated in IBD and most likely serves to recruit alpha ₄ beta ₇-expressing lymphocytes to the region. In vivo studies involving nonobese diabetic (NOD) mice have also suggested that MAdCAM-1/ alpha ₄ beta ₇ interaction plays a role in diabetes development in this model. Mouse MAdCAM-1 is a 405 amino acid (aa) residue protein with a 21 aa signal sequence, a 344 aa extracellular domain, a 20 aa transmembrane domain and a 20 aa cytoplasmic domain.

• References:

1. Briskin, M.J. et al. (1993) Nature 363:461.

2. Yang, X.D. et al. (1997) Diabetes 46:1542.

3. Sampaio, S.O. et al. (1995) J. Immunol. 155:2477.

4. Kraal, G. et al. (1995) Am. J. Pathol. 147:763.

5. Berg, E.L. et al. (1993) Nature 366:695.

6. Takeuchi, M. and V.R. Baichwal (1995) Proc. Natl. Acad. Sci. USA 92:3561.

• Long Name:

  Mucosal Addressin Cell Adhesion Molecule 1

• Entrez Gene IDs:

  8174 (Human); 17123 (Mouse)

• Alternate Names:

  hMAdCAM-1; MACAM1; MAdCAM1; MAdCAM-1; mucosal addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule-1; mucosal vascular addressin cell adhesion molecule 1