• Species Reactivity

  Human

• Specificity

  Detects human NTB‑A/SLAMF6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) BLAME and rhCRACC is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 292810

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human NTB‑A/SLAMF6    
  Leu28-Lys225    
  Accession # Q96DU3

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human NTB-A/SLAMF6 Fc Chimera (Catalog # ) under non-reducing conditions only

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NTB-A/SLAMF6

NTB-A (NK-T-B-antigen), also known as Ly108 and SLAMF6, is a 60 kDa type I transmembrane glycoprotein that belongs to the SLAM subgroup of the CD2 family (1). Mature human NTB-A contains a 205 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set and one Ig-like C2-set domain. It also contains a 21 aa transmembrane segment and an 84 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs (ITSMs) (2, 3). An alternately spliced isoform is truncated in the cytoplasmic domain and lacks the two ITSMs. Within the ECD, human NTB-A shares 48% aa sequence identity with mouse and rat NTB-A. The ECD of human NTB-A shares 19%‑34% aa sequence identity with comparable regions of human 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, and SLAM. NTB-A is expressed on the surface of NK, T, and B lymphocytes as well as eosinophils (2, 4, 5). It interacts homophilically through weak associations between the Ig-V domains (2, 5‑7). NTB-A functions as an activating coreceptor on NK and T cells (2, 5, 6, 8). Tyrosine phosphorylation in the membrane proximal ITSM enables specific association with EAT-2, an interaction that is required for NTB-A mediated cytotoxicity of NK cells (9). Phosphorylation-dependent NTB-A association with SAP is required for full production of IFN-gamma by NK cells (5, 9). This interaction is independent of EAT-2 binding and appears to involve the membrane distal ITSM (5, 9). NTB-A deficient mice show weakened Th2 responses and elevated levels of neutrophil-derived inflammatory mediators (10). On B cells, NTB-A modulates immunoglobulin class switching and the balance between tolerance and autoimmunity (5, 11).

• References:

1. Veillette, A. (2006) Immunol. Rev. 214:22.

2. Bottino, C. et al. (2001) J. Exp. Med. 194:235.

3. Fraser, C.C. et al. (2002) Immunogenetics 53:843.

4. Munitz, A. et al. (2005) J. Immunol. 174:110.

5. Valdez, P.A. et al. (2004) J. Biol. Chem. 279:18662.

6. Flaig, R.M. et al. (2004) J. Immunol. 172:6524.

7. Cao, E. et al. (2006) Immunity 25:559.

8. Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.

9. Eissmann, P. and C. Watzl (2006) J. Immunol. 177:3170.

10. Howie, D. et al. (2005) J. Immunol. 174:5931.

11. Kumar, K.R. et al. (2006) Science 312:1665.

• Long Name:

  NK-T-B Antigen/SLAM Family Member 6

• Entrez Gene IDs:

  114836 (Human)

• Alternate Names:

  Activating NK receptor; CD352 antigen; CD352; KALIFLJ50657; Ly108; NK-T-B-antigen; NTBA receptor; NTBA; NTB-A; NTB-AMGC104953; NTBAT- and B-cell antigen; SF2000; SLAM family member 6; SLAMF6