详细说明
Species Reactivity
Human
Specificity
Detects human Progesterone R B in Western blots.
Source
Monoclonal Mouse IgG 2B Clone # 569012
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Progesterone R B
Met1-Leu189
Accession # P06401Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Human Progesterone R B/NR3C3 by Western Blot. Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Progesterone R B/NR3C3 Monoclonal Antibody (Catalog # MAB5886) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for Progesterone R B/NR3C3 at approximately 118 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Immunocytochemistry | Progesterone R B/NR3C3 in T47D Human Cell Line. Progesterone R B/NR3C3 was detected in immersion fixed T47D human breast cancer cell line using Mouse Anti-Human Progesterone R B/NR3C3 Monoclonal Antibody (Catalog # MAB5886) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for . |
Immunohistochemistry | Progesterone R B/NR3C3 in Human Prostate Cancer Tissue. Progesterone R B/NR3C3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human Progesterone R B/NR3C3 Monoclonal Antibody (Catalog # MAB5886) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # ). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for . |
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Progesterone R B/NR3C3
Progesterone Receptor B (PR-B) is a 118 kDa member of the NR3 subfamily within the nuclear hormone receptor family of proteins. It is expressed in several tissues, including female reproductive organs as well as neurons throughout the CNS. PR-B is particularly important in the mammary gland where it mediates proliferative responses to progesterone. Human PR-B contains an N-terminal regulatory region (aa1‑566), a DNA binding domain (aa 567‑639), and a steroid-binding region (aa 681‑933). Ligand binding induces a key phosphorylation event at Ser294 by ERK1/2.
Long Name:
Progesterone Receptor B
Entrez Gene IDs:
5241 (Human)
Alternate Names:
PGRB; Progesterone R B; Progesterone RB
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