• Species Reactivity

  Human

• Specificity

  Detects human IL-6 R alpha in direct ELISAs.

• Source

  Recombinant Monoclonal Mouse IgG    ₁ Clone # 556704R

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  E. coli-derived recombinant human IL-6 R alpha    
  Arg387-Arg468    
  Accession # P08887

• Formulation

  Supplied as a solution in PBS. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunocytochemistry

  3-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C, as supplied.

• 1 month, 2 to 8 °C under sterile conditions after opening.

• 6 months, -20 to -70 °C under sterile conditions after opening.

Background: IL-6 R alpha

The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex (1‑4). Within the extracellular domain, human IL‑6 R alpha shares 52% aa sequence identity with mouse and rat IL‑6 R alpha.

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL‑6/IL‑6 R alpha complex to membrane-bound gp130 (6, 7).

• References:

1. Yamasaki et al. (1988) Science 241:825.

2. Baumann et al. (1990) J. Biol. Chem. 265:19853.

3. Hibi et al. (1990) Cell 63:1149.

4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.

5. Novick et al. (1989) J. Exp. Med. 170:1409.

6. Yasukawa et al. (1990) J. Biochem. 108:673.

7. Saito et al. (1991) J. Immunology 147:168.

• Long Name:

  Interleukin 6 Receptor alpha

• Entrez Gene IDs:

  3570 (Human); 16194 (Mouse)

• Alternate Names:

  CD126; IL-6 R alpha; IL6R alpha; IL6R; IL-6Ra