• Species Reactivity

  Mouse

• Specificity

  Detects mouse FCRN in direct ELISAs and Western blots. In direct ELISAs, approximately 6% cross-reactivity with recombinant human FCRN is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived mouse FCRN    
  Ser22-Ser301    
  Accession # AAH03786

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.25 µg/mL

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Intracellular Staining by Flow Cytometry

  2.5 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.2 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRN

FcRn (IgG receptor FcRn large subunit p51/ alpha -chain) is a 44-52 kDa member of the Ig superfamily. In mouse, it is expressed by intestinal epithelium of neonates and fetal yolk sac endoderm during pregnancy. Together with p14/ beta ₂-Microglobulin, FcRn forms an MHC I-like noncovalent heterodimer termed the FcRn complex. In rodent, FcRn transports IgG in milk into the intestine, and IgG from the mother's blood into the mouse via endodermal FcRn linked to a special Fc gamma RIIb macrophage receptor. It is also expressed by select macrophages and DCs, and participates in endosomal-associated antigen presentation to CD4+ T cells. Mature mouse FcRn is a 344 amino acid (aa) type I transmembrane glycoprotein. It contains a 276 aa extracellular region (aa 22-297) that contains an Ig-like domain (aa 201-288). The FcRn complex forms a homodimer when binding IgG. One potential splice variant shows a four aa insertion after Gly109. Over aa 22-301 of the splice variant (GenBank #:AAH03786), mouse FcRn shares 91% and 68% aa identity with rat and human FcRn, respectively.

• Long Name:

  Neonatal Fc Receptor

• Entrez Gene IDs:

  2217 (Human); 14132 (Mouse); 29558 (Rat)

• Alternate Names:

  alpha-chain; Fc fragment of IgG, receptor, transporter, alpha; FCGRT; FcRn alpha chain; FCRN; FCRNimmunoglobulin receptor, intestinal, heavy chain; IgG Fc fragment receptor transporter alpha chain; IgG receptor FcRn large subunit p51; major histocompatibility complex class I-like Fc receptor; Neonatal Fc receptor; neonatal Fc-receptor for Ig