• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse Myocardin in Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 355521

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Myocardin    
  Met97-Leu290    
  Accession # Q8IZQ8

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myocardin

Myocardin (MYOCD) is a transcriptional co-activator necessary for differentiation of smooth muscle cells. MYOCD functions by binding the transcription factor Serum Response Factor (SRF) and stimulating smooth muscle cell-specific gene expression.

• Entrez Gene IDs:

  93649 (Human); 214384 (Mouse); 246297 (Rat)

• Alternate Names:

  BSAC2A; MYCD; Myocardin; MYOCD; Srfcp