Preparation and Storage

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

• 12 months from date of receipt, 2 to 8 °C as supplied.

• 3 months, 2 to 8 °C under sterile conditions after opening.

Background: HR23A/Rad23A TUBE1

Human HR23A/Rad23A has two Ubiquitin-associated (UBA) motifs that can each bind Ubiquitin via a hydrophobic surface formed by residues located within the alpha 1 and alpha 3 helices of each UBA domain (1,2). Tandem-repeated Ubiquitin binding entities (TUBEs), which consist of multiple tandem Ubiquitin-binding UBA motifs, have been developed for the isolation and identification of ubiquitinated proteins (3). TUBEs show increased affinity for poly-Ubiquitin moieties compared to single UBA motifs (4). Additionally, TUBEs protect polyubiquitinated proteins from deubiquitylating enzymes, allowing for the detection of polyubiquitinated proteins at relatively low levels of abundance (5).

This affinity resin can be used for the enrichment, isolation and identification of K63-linked (preferentially) or K48-linked poly-Ub chains or ubiquitinated substrates that contain these linkages.

• References:

1. Chen, L. et al. (2001) EMBO Rep. 2:933.

2. Wang, Q. et al. (2003) Biochemistry 42:13529.

3. Hjerpe, R. et al. (2009) EMBO Rep. 10:1250.

4. Hurley, J.H. et al. (2006) Biochem. J. 399:361.

5. Lopitz-Otsoa, F. et al. (2010) Biochem. Soc. Trans. 38:40.

• Long Name:

  RAD23 Homolog A

• Entrez Gene IDs:

  5886 (Human); 19358 (Mouse); 361381 (Rat)

• Alternate Names:

  HR23A; Rad23A