• Purity

  >80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

• Activity

  Recombinant Human USP1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1 concentration of 0.01-0.5 μM.

• Source

  Spodoptera frugiperda,     Sf 21 (baculovirus)-derived Contains substitutions at G670A & G671A and a C-terminal 6-His tag

• Accession #

• Predicted Molecular Mass

  89 kDa

Background: USP1

USP1 (Ubiquitin-Specific-processing Protease 1, also known as Ubiquitin carboxyl-terminal hydrolase 1) is a deubiquitinating enzyme of the C19 peptidase family and functions as a negative regulator of the Fanconi Anemia pathway. Reported substrates of USP1 include monoubiquitinated FANCD2 and monoubiquitinated PCNA. USP1 plays important roles in DNA damage responses and cancer-related processes, and inhibiting the function of this deubiquitinase sensitizes some cancer cells to chemotherapy. By itself, USP1 is nearly completely inactive and requires a protein binding partner, UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) to stimulate its deubiquitinase activity. In addition to the naturally occurring substates listed above, the USP1/UAF1 complex has been demonstrated to cleave K6, K33, and K63-linked polyubiquitin chains. This recombinant protein contains a C-terminal 6-His tag, and glycine-to-alanine substitutions at positions 670 and 671 (UniProt O94782 numbering).

• References:

1. Cohn M.A., et al. (2007) Mol. Cell 28:786-797

2. Huang T.T., et al. (2006) Nat. Cell Biol. 8: 339-347

3. Nijman S.M.B., et al. (2005) Mol. Cell 17:331-339

4. Williams S.A, et al. (2011) Cell 146: 918–930

• Long Name:

  Ubiquitin Specific Protease 1

• Entrez Gene IDs:

  7398 (Human); 230484 (Mouse); 313387 (Rat)

• Alternate Names:

  Deubiquitinating Enzyme 1; EC 3.1.2.15; EC 3.4.19.12; hUBP; ubiquitin carboxyl terminal hydrolase 1; Ubiquitin Carboxyl-Terminal Hydrolase 1; ubiquitin specific peptidase 1; ubiquitin specific processing protease 1; ubiquitin specific protease 1; Ubiquitin Thioesterase 1; Ubiquitin thiolesterase 1; Ubiquitin-specific-processing protease 1; UBP; USP1