Mouse S1P1/EDG-1 Biotinylated MAb (Clone 713412) 100 TESTS

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Mouse S1P1/EDG-1 Biotinylated MAb (Clone 713412) 100 TESTS信息二维码

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  • 更新2024-11-26 20:30
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产品介绍

    基本参数

    详细说明

    • Species Reactivity

      Mouse

    • Specificity

      Detects mouse S1P    1/EDG‑1 peptide in direct ELISAs.

    • Source

      Monoclonal Rat IgG    2A Clone # 713412

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      Mouse S1P    1/EDG‑1 synthetic peptide (T4-H28)    
      Accession # O08530

    • Formulation

      Supplied in a saline solution containing BSA and Sodium Azide.

    • Label

      Biotin

    Applications

    • Recommended    
      Concentration

      Sample

    • Flow Cytometry

      10 µL/10    6 cells

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Flow Cytometry      
         

    Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and eGFP was stained with Rat Anti-Mouse S1P1/EDG‑1 Biotinylated Monoclonal Antibody (Catalog # FAB7089B) followed by Streptavidin-Allophycocyanin (Catalog # ). Quadrant markers were set based on control antibody staining (Catalog # ).

    Flow Cytometry      
         

    Detection of S1P1/EDG‑1 in Mouse thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse S1P1/EDG‑1 Biotinylated Monoclonal Antibody (Catalog # FAB7089B) followed by Streptavidin-Allophycocyanin (Catalog # ) and Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # ). Quadrant markers were set based on control antibody staining (Catalog # ).

    Preparation and Storage

    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

    • Stability & Storage

      Do not freeze.    

      • 12 months from date of receipt, 2 to 8 °C as supplied.

    Background: S1P1/EDG-1

    S1P  1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P  1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P  1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

    • Long Name:

      Endothelial Differentiation Gene 1

    • Entrez Gene IDs:

      1901 (Human); 13609 (Mouse)

    • Alternate Names:

      CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1











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