• Species Reactivity

  Human

• Specificity

  Detects human TRA‑1‑85.

• Source

  Monoclonal Mouse IgG    ₁ Clone # TRA-1-85

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  2120Ep human embryonal carcinoma cell line

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

• Label

  Biotin

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  Williams, B.P. et al. (1988) Immunogenetics. 27:322. This application was not tested by R&D Systems.

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• Flow Cytometry

  2.5 µg/10    ⁶ cells

  Human peripheral blood mononuclear cells

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• Immunocytochemistry

  Chen, Y.     et al. (2003) Cell Res.     13:251. This application was not tested by R&D Systems.

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRA-1-85/CD147

The TRA-1-85 clone recognizes the cell surface expressed Oka blood group antigen in almost all human cell types (1). This antibody is frequently used to identify human cells.

• References:

1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.

• Entrez Gene IDs:

  682 (Human)

• Alternate Names:

  OKa Blood Group Antigen; TRA185; TRA-1-85