• Species Reactivity

  Human

• Specificity

  Detects human BMP‑15/GDF‑9B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human BMP-1.1, -2, -3, -3b, -4, -5, -6, -7, -8, or -10 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 317809

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human BMP‑15/GDF‑9B    
  Gln268-Arg392    
  Accession # O95972

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human BMP‑15/GDF‑9B (Catalog # )

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-15/GDF-9B

Bone morphogenetic protein 15 (BMP-15), also known as GDF-9B, is a TGF-beta superfamily ligand that is expressed by oocytes throughout folliculogenesis, and plays an important role in oocyte development (1). BMP-15 promotes the FSH-independent proliferation of ovarian granulosa cells (GC) and induces GC glycolysis and cholesterol synthesis (2‑4). It also induces GC production of stem cell factor which, in turn, negatively regulates BMP-15 expression in oocytes (5). BMP-15 blocks the FSH-induced GC expression of FSH R and multiple steroidogenic molecules (6). BMP-15 is synthesized with a 249 amino acid (aa) N-terminal propeptide (7). The propeptide is cleaved intracellularly from the 50 kDa proBMP-15 but remains associated with mature BMP-15 (8). Mature BMP-15 exists in 16 kDa and 17 kDa forms which are distinguishable by the presence of O-linked glycosylation on the 17 kDa form (8). Mature BMP-15 is phosphorylated, a modification which is required for the stimulation of GC proliferation (9). BMP-15 exerts its effects through interactions with BMPR-IB/ALK6 and BMPR-II (9‑11). Mature BMP-15 forms 34 kDa noncovalently-linked homodimers and 37 kDa heterodimers with mature GDF-9 (12). Both of these oocyte-expressed factors lack the cysteine that mediates disulfide-linked dimerization in most TGF-beta superfamily ligands (1). Although heterodimerization with GDF-9 may limit the secretion of active BMP-15, these two factors synergize in promoting oocyte survival and folliculogenesis (12, 13). Mature human BMP-15 shares 70%, 68%, and 78% aa sequence identity with mouse, rat, and sheep BMP-15, respectively. It shares 27%‑38% aa sequence with other BMPs.

• References:

1. Moore, R.K. and S. Shimasaki (2005) Mol. Cell. Endocrinol. 234:67.

2. Otsuka, F. et al. (2000) J. Biol. Chem. 275:39523.

3. Sugiura, K. et al. (2007) Development 134:2593.

4. Su, Y.-Q. et al. (2008) Development 135:111.

5. Otsuka, F. and S. Shimasaki (2002) Proc. Natl. Acad. Sci. 99:8060.

6. Otsuka, F. et al. (2001) J. Biol. Chem. 276:11387.

7. Dube, J.L. et al. (1998) Mol. Endocrinol. 12:1809.

8. Saito, S. et al. (2008) Prot. Sci. 17:362.

9. McMahon, H.E. et al. (2008) Endocrinology 149:812.

10. Moore, R.K. et al. (2003) J. Biol. Chem. 278:304.

11. Edwards, S.J. et al. (2008) Endocrinology 149:1026.

12. Liao, W.X. et al. (2003) J. Biol. Chem. 278:3713.

13. Yan, C. et al. (2001) Mol. Endocrinol. 15:854.

• Long Name:

  Bone Morphogenetic Protein 15

• Entrez Gene IDs:

  9210 (Human); 12155 (Mouse); 59302 (Rat)

• Alternate Names:

  BMP15; BMP-15; bone morphogenetic protein 15; GDF-9B; GDF9BODG2; Growth/differentiation factor 9B; POF4