详细说明
Species Reactivity
Mouse
Specificity
Detects mouse OSM R beta.
Source
Monoclonal Rat IgG 2A Clone # 118125
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse OSM R beta
Glu24-Leu738
Accession # O70458Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin
Applications
Recommended
ConcentrationSample
Flow Cytometry
2.5 µg/10 6 cells
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed frozen sections of mouse embryo (E13.5-15.5)
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of OSM R beta in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse OSM R beta Biotinylated Monoclonal Antibody (Catalog # BAM662, filled histogram) or isotype control antibody (Catalog # , open histogram), followed by Streptavidin-Phycoerythrin (Catalog # ). |
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: OSM R beta
Oncostatin M (OSM) is a member of the IL-6 family of cytokines that share the gp130 as a common signal transducing receptor subunit. Human OSM signals through two types of human OSM receptor complexes: the type I complex comprising the Leukemia Inhibitory Factor Receptor beta (LIF R beta ) and gp130, the type II complex made up of OSM Receptor beta (OSM R beta ) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Mouse OSM R beta cDNA encodes a 971 amino acid (aa) residue type I transmembrane protein which contains a 23 aa residue signal peptide, an extracellular domain of 714 aa, a transmembrane domain of 20 aa and a 214 aa cytoplasmic domain. Mouse OSM R beta alone binds mOSM with low-affinity, but forms a high-affinity binding complex in the presence of gp130. Mouse OSM R beta is 55% identical at the amino acid sequence level to human OSM R beta.
References:
Lindberg, R.A. et al. (1998) Mol. Cell. Biol. 18:3357.
Tanaka, M. et al. (1999) Blood 93:804.
Long Name:
Oncostatin M Receptor beta
Entrez Gene IDs:
9180 (Human); 18414 (Mouse); 310132 (Rat)
Alternate Names:
OSM R beta; OSMR; OSMRB