• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse Frizzled-1, -2, -3, -6, -7, or -8 is observed. In flow cytometry and immunohistochemistry, this antibody also detected Frizzled-4 on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3, and also on human HEK293 cells.

• Source

  Monoclonal Rat IgG    _2A Clone # 145901

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4    
  Met1-Glu180    
  Accession # Q61088

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

• Endotoxin Level

  <0.15 EU per 1 μg of the antibody by the LAL method.  

• Label

  Biotin

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Frizzled-4 (Catalog # )    
  Recombinant Mouse Frizzled-4 Fc Chimera (Catalog # )

• 
  

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  HEK293 human embryonic kidney cell line

• 
  

• Immunocytochemistry

  8-25 µg/mL

  Immersion fixed HEK293 human embryonic kidney cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-4

Frizzled-4 belongs to the Frizzled family of 7-pass transmembrane proteins and binds to Wnt-8. It is implicated in several developmental processes, notably in retinal angiogenesis.

• Entrez Gene IDs:

  8322 (Human); 14366 (Mouse)

• Alternate Names:

  CD344 antigen; CD344; EVR1; exudative vitreoretinopathy 1; FEVR; frizzled (Drosophila) homolog 4; frizzled homolog 4 (Drosophila); Frizzled4; Frizzled-4; Fz-4; FZD4; FZD4S; FzE4; GPCR; hFz4; MGC34390; WNT receptor frizzled-4