• Species Reactivity

  Human

• Specificity

  Detects human Stanniocalcin 1/STC‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Stanniocalcin 2 or recombinant mouse Stanniocalcin 1 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 380715

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Stanniocalcin 1/STC‑1    
  Thr18-Ala247    
  Accession # P52823

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.05 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stanniocalcin 1/STC-1

Stanniocalcin-1 (STC-1) is a secreted 35 kDa disulfide-linked homodimer that is related to a calcium-regulating hormone in fish. Mature human Stanniocalcin-1 is 230 aa in length, and contains five intrachain disulfide bonds plus one interchain disulfide bond. Circulating Stanniocalcin-1 is both glycosylated and phosphorylated. Mature human Stanniocalcin-1 is 98%, 97%, 95% and 97% aa identical to mature canine, mouse, bovine and rat Stanniocalcin-1, respectively. Stanniocalcin-1 promotes phosphorus absorption and blocks chemokine-induced chemotaxis.

• Entrez Gene IDs:

  6781 (Human)

• Alternate Names:

  Stanniocalcin 1; stanniocalcin-1; STC1; STC-1; STCSTC-1