• Species Reactivity

  Human

• Specificity

  Detects human Carboxypeptidase A2/CPA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CPA1, rhCPA4, rhCPB1, or rhCPE is observed. Recognizes the pro form (aa 17‑417), but not the activated enzyme (aa 113‑417) in Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 384023

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A2/CPA2    
  Leu18-His417    
  Accession # P48052

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Carboxypeptidase A2/CPA2 (Catalog # )

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase A2/    
  CPA2 (Catalog # ),

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxypeptidase A2/CPA2

Carboxypeptidase A2 encoded by the CPA2 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic side chains including Phe, Tyr, and Trp. The deduced amino acid sequence of human CPA2 consists of a signal peptide (aa 1 to 16), a pro region (aa 17 to 112), and a mature chain (aa 113 to 417). The amino acid sequence of human CPA2 is 99.5%, 90%, 87%, 86% and 70% identical to that of chimpanzee, canine, rat, mouse and chicken.

• References:

1. Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 821, Academic Press, San Diego.

• Entrez Gene IDs:

  1358 (Human)

• Alternate Names:

  carboxypeptidase A2 (pancreatic); Carboxypeptidase A2; CPA2; EC 3.4.17; EC 3.4.17.15