• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to compete with Biotinylated Recombinant Human Ephrin‑A5 Fc Chimera (Catalog # ) for binding to immobilized recombinant mouse Eph-A3 Fc Chimera in a functional ELISA assay.    
     Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Mouse myeloma cell line, NS0-derived

  Human Ephrin-A5 (Gln21-Asn203) Accession # P52803	IEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln21 predicted    
   

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  48.6 kDa (monomer)

• SDS-PAGE

  50-55 kDa, reducing conditions

Background: Ephrin-A5

Ephrin‑A5, also known as AL‑1, RAGS, and LERK‑7, is an approximately 25 kDa member of the Ephrin‑A family of GPI‑anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin‑A ligands are structurally related to the extracellular domains of the transmembrane Ephrin‑B ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Ephrin‑A5 preferentially interacts with receptors in the EphA family but also with EphB2 (3). Mature human Ephrin‑A5 shares 99.5% aa sequence identity with mouse and rat Ephrin‑A5, respectively (4). Ephrin‑A5 is expressed in multiple tissues during development, particularly in the brain (5, 6). It can exert repulsive or attractive effects on migrating neurons in the developing brain and motor column of the spinal cord (6‑10). Ephrin‑A5 repels migrating axons by inducing growth cone collapse and neurite retraction and by inhibiting the neurotrophic effects of NGF and BDNF (3, 11, 12). It interacts   in cis with EphA3 on retinal axon growth cones which reduces axonal sensitivity to Ephrin‑A5   in trans (13). In the adult, Ephrin‑A5 is expressed on hippocampal neurons and astrocytes and induces the development of hippocampal synapses (9, 14, 15). It supports the proliferation of neural progenitors and the survival of newly differentiated neurons (14). Ephrin‑A5 also functions as a tumor suppressor (16). Its normal function in inhibiting EGFR signaling is compromised by its down‑regulation in glioma (16). Ephrin‑A5 is also expressed by muscle precursor cells and interacts with EphA4 to restrict their migration to the correct locations during forelimb morphogenesis (17).

• References:

1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.

2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.

3. Himanen, J.-P. et al. (2004) Nat. Neurosci. 7:501.

4. Winslow, J.W. et al. (1995) Neuron 14:973.

5. Deschamps, C. et al. (2010) BMC Neurosci. 11:105.

6. Cooper, M.A. et al. (2009) Dev. Neurobiol. 69:36.

7. Frisen, J. et al. (1998) Neuron 20:235.

8. Zimmer, G. et al. (2008) Eur. J. Neurosci. 28:62.

9. Otal, R. et al. (2006) Neuroscience 141:109.

10. Eberhart, J. et al. (2004) J. Neurosci. 24:1070.

11. Munoz, L.M. et al. (2005) Dev. Biol. 283:397.

12. Meier, C. et al. (2011) PLoS ONE 6:e26089.

13. Carvalho, R.F. et al. (2006) Nat. Neurosci. 9:322.

14. Hara, Y. et al. (2010) Stem Cells 28:974.

15. Akaneya, Y. et al. (2010) PLoS ONE 5:e12486.

16. Li, J.-J. et al. (2009) Oncogene 28:1759.

17. Swartz, M.E. et al. (2001) Development 128:4669.

• Entrez Gene IDs:

  1946 (Human); 13640 (Mouse)

• Alternate Names:

  AF1; AL-1; EFL-5; EFNA5; EPH-related receptor tyrosine kinase ligand 7; EphrinA5; Ephrin-A5; EPLG7; GLC1M; LERK-7; LERK7EFL5; RAGS