• Purity

  >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

• Activity

  Reaction conditions will need to be optimized for each specific application.  We recommend an initial recombinant human USP11 concentration of 10-50 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (     U-550,      U-555).

• Source

  Spodoptera frugiperda,     Sf 21 (baculovirus)-derived Contains an C-terminal 6-His tag

• Accession #

• Predicted Molecular Mass

  111 kDa

Background: USP11

Ubiquitin carboxyl-terminal hydrolase 11 (USP11) is a specialized cysteine protease with a predicted molecular weight of 110 kDa. USP11 is a member of the peptidase C19 family and the human protein shares 77% amino acid sequence identity with its mouse ortholog. USP11 plays a role in DNA double-strand break (DSB) repair, and cells are hypersensitive to gamma radiation when USP11 has been knocked down. USP11 overexpression is often observed in colorectal cancer and melanoma and is correlated with poor survival. In these cases USP11 may be driving disease states by stabilizing cIAP2. Interestingly, recent genetic analysis suggests that USP11 was generated during the course of vertebrate evolution by a small-scale duplication of the USP4-encoding region. This recombinant protein contains a C-terminal 6-His tag.

• References:

1. Lee, E.W. et al. (2015) Cell Death and Differ.  22: 1463

2. Vlasschaert, C. et al. (2015) BMC Evol. Biol. 15: 230

3. Yu, M. et al. (2016) J.Biol.Chem. 291: 959

• Long Name:

  Ubiquitin Specific Protease 11

• Entrez Gene IDs:

  8237 (Human); 236733 (Mouse); 408217 (Rat)

• Alternate Names:

  Deubiquitinating enzyme 11; EC 3.1.2.15; EC 3.4.19.12; ubiquitin carboxyl-terminal hydrolase 11; ubiquitin specific peptidase 11; ubiquitin specific protease 11; Ubiquitin Thioesterase 11; Ubiquitin thiolesterase 11; ubiquitin-specific processing protease 11; Ubiquitin-specific-processing protease 11; UHX1; UHX1ubiquitin carboxyl-terminal hydrolase, X-linked; USP11