• Species Reactivity

  Human

• Specificity

  Detects human Neprilysin/CD10 in Western blots and direct ELISAs. In Western blots, less than 5% cross-reactivity with recombinant human ECE-2 and recombinant mouse Neprilysin is observed.

• Source

  Monoclonal Mouse IgG2b Clone # 212504

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human Neprilysin/CD10    
  Met45-Pro743    
  Accession # P08473

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Daudi human Burkitt's lymphoma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neprilysin/CD10

Neprilysin (NEP) is also known as CALLA and CD10. It is a zinc metalloprotease expressed at the cell surface that cleaves a variety of bioactive peptides such as enkephalins, atrial natriuretic peptide and amyloid beta peptide.

• Entrez Gene IDs:

  4311 (Human); 17380 (Mouse); 24590 (Rat)

• Alternate Names:

  Atriopeptidase; CALLA; CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); CD10 antigen; CD10; CD10); CD10membrane metallo-endopeptidase variant 1; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24; EC 3.4.24.11; Enkephalinase; EPN; Leu-19; membrane metallo-endopeptidase variant 2; membrane metallo-endopeptidase; MGC126681; MGC126707; MME; NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA; Neprilysin; Neutral Endopeptidase 24.11; Neutral endopeptidase; NKH1; SFE; Skin fibroblast elastase