• Species Reactivity

  Human

• Specificity

  Detects human DEP‑1/CD148.

• Source

  Monoclonal Mouse IgG2b Clone # 261922

• Immunogen

  E. coli-derived recombinant human DEP‑1/CD148    
  Arg997-Ala1337    
  Accession # Q12913

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 405

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Human whole blood

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DEP-1/CD148

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kilodalton) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T-cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta‑catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.

• References:

1. Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.

2. Sorby, M. et al. (2001) Oncogene 20:5219.

3. Jandt, E. et al. (2003) Oncogene 22:4175.

4. Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.

5. Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.

6. Borges, L.G. et al. (1996) Circ. Res. 79:570.

7. de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.

8. Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.

9. Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.

10. Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.

11. Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.

12. Holsinger, L.J. et al. (2002) Oncogene 21:7067.

• Long Name:

  Density Enhanced Receptor Protein Tyrosine Phosphatase

• Entrez Gene IDs:

  5795 (Human); 19271 (Mouse); 29645 (Rat)

• Alternate Names:

  CD148 antigen; CD148; Density-enhanced phosphatase 1; DEP1; DEP-1; DEP1EC 3.1.3.48; HPTP eta; HPTPeta; HPTP-eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; protein tyrosine phosphatase, receptor type, J; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; receptor-type tyrosine-protein phosphatase eta; R-PTP-ETA; R-PTP-J; SCC1; susceptibility to colon cancer 1, mouse, homolog of