• Species Reactivity

  Human

• Specificity

  Detects human Persephin in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) AgRP, rhArtemin, rh beta IG-H3, rhCripto-1, recombinant drosophila Decapentaplegic, rhGDNF, rhLAP, rhLatent TGF-beta 1, rhLefty-A, rhMIS, rhNeurturin, recombinant mouse (rh) Nodal, rmPersephin, rhTGF-alpha, rhTGF-beta 1, rhTGF-beta 1.2, rhTGF-beta 2, rhTGF-beta 3, or recombinant amphibian TGF-beta 5 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 275508

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Persephin    
  Ala61-Gly156    
  Accession # O60542

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Persephin (Catalog # )

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• Immunohistochemistry

  8-25 µg/mL

  Immersion fixed paraffin-embedded sections of human brain (cortex) and spinal cord

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Persephin

Persephin is a secreted protein belonging to the glial cell line-derived neurotrophic factor (GDNF) family of the TGF-beta superfamily. It shares 38-46% amino acid (aa) identity with family members GDNF, neurturin and artemin. Persephin is expressed at very low levels in most tissues (1). The 156 aa, 10-12 kDa mature protein contains a signal sequence, a pro-domain and a 96 aa mature sequence with several cysteines that are conserved among family members. It circulates as an unglycosylated disulfide-linked homodimer. Mature human Persephin shares 81% and 80%, 89%, and 87% amino acid sequence identity with mouse, rat, bovine and canine Persephin, respectively. Like other GDNF family members, Persephin acts through engagement of GRF alpha 4, a glycosylphosphatidylinositol (GPI)-linked GDNF receptor family (GRF) member that signals through the receptor tyrosine kinase RET. Persephin is reported to promote both the survival and growth of central dopaminergic and motor neurons, and kidney development (1). These effects are correlated with the expression patterns of GFR alpha 4, and RET (2, 3). Functional GFR alpha 4 isoforms are found only in thyroid, adrenal medulla and portions of the central nervous system, and include GPI-linked, transmembrane and soluble forms (3, 4). In vitro, Persephin promotes survival only in neurons which coexpress GPI-linked GFR alpha 4 with RET (2, 5). This effect does not show a strong correlation to the recruitment of RET in lipid rafts seen with other GDNF family members (6). Disruption of the Persephin gene results in mice that are morphologically normal but have more damage and less effective repair after central nervous system insult that stimulates a stroke. Microinjection of Persephin prior to treatment protects against damage in both wild-type and mutant mouse brains, but surprisingly, high doses of Persephin are detrimental (7).

• References:

1. Milbrandt, J. et al. (1998) Neuron 20:245.

2. Lindahl, M. et al. (2001) J. Biol. Chem. 276:9344.

3. Lindahl, M. et al. (2000) Mol. Cell. Neurosci. 15:522.

4. Akerud, P. et al. (2002) Mol. Cell. Neurosci. 21:205.

5. Enokido, Y. et al. (1998) Current Biol. 8:1019.

6. Yang, J. et al. (2004) FEBS Lett. 569:267.

7. Tomac, A.C. et al. (2002) Proc. Natl. Acad. Sci. USA 99:9521.

• Entrez Gene IDs:

  5623 (Human); 19197 (Mouse)

• Alternate Names:

  Persephin; PSP; PSPN