• Species Reactivity

  Human

• Specificity

  Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J.     et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W.     et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S.     et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).

• Source

  Monoclonal Mouse IgG    ₁ Clone # UCHT1

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human thymocytes (1)    
   

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD3 epsilon

CD3 epsilon is a key signaling molecule that participates in the formation of the T Cell Receptor (TCR). The alpha beta TCR is composed of six distinct subunits. There is an alpha -beta disulfide-linked heterodimer that recognizes antigen, and this is accompanied by four CD3 subunits that transduce an intracellular signal. CD3 epsilon forms a heterodimer with both CD3 gamma and CD3δ, creating two signaling complexes. These two heterodimers are supplemented by a CD3 zeta zeta homodimer that lies in the center of the TCR complex, generating a fully-functional TCR (2). 

• References:

1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.

2. Call, M.E. and K.W. Wucherpfennig (2005) Annu. Rev. Immunol. 23: 101

• Entrez Gene IDs:

  916 (Human); 12503 (Mouse)

• Alternate Names:

  CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE