详细说明
Specificity
Stains N-terminal,C-terminal, and Internal Sequence HA-tagged protein transfectants but not irrelevant transfectants in flow cytometry.
Source
Recombinant Monoclonal Mouse IgG 1 Clone # 912426
Purification
Protein A or G purified from cell culture supernatant
Immunogen
HA Tag peptide
Accession # ABB51961Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
5 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry | Detection of HA Tag in HEK293 Human Cell Line Transfected withHA-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) HA-tagged proteins (filled histogram) or (B) irrelevant transfectants (open histogram) was stained with Mouse Anti-HA Tag Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # IC6875T). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: HA Tag
Entrez Gene IDs:
ABB51961.1 (Viral)
Alternate Names:
HA Epitope Tag; HA Tag; HA; Hemagglutinin