• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

• Activity

  Recombinant Human His6-Apg3p/ATG3/Apg3L is a member of the Apg-conjugating (E2) enzyme family that receives Apg from a Apg-activating (E1) enzyme and subsequently interacts with a Apg ligase (E3) to conjugate Apg to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Apg3p/ATG3/Apg3L concentration of 0.1-1 μM.

• Source

  E. coli-derived Contains a C-terminal 6-His tag

• Accession #

• Predicted Molecular Mass

  37 kDa

Background: ATG3

Autophagy-related 3 (ATG3), also known as Apg3L and Apg3p, functions as a regulatory component of autophagosome biogenesis necessary for autophagy. It is a 314 amino acid (aa) protein with a predicted molecular weight of 36 kDa. ATG3 exhibits 98% aa sequence identity with both its mouse and rat orthologs. It is a Ubiquitin-conjugating (E2)-like enzyme that works in concert with Atg12-Atg5, a Ubiquitin Ligase (E3)-like enzyme conjugate, to catalyze the transfer of Atg8 (LC3, GATE-16, and GABARAP in mammals) to phosphatidylethanolamine (PE) in the developing autophagosome membrane (1-4). This lipidation of Atg8 proteins is necessary for autophagosome maturation (5).

• References:

1. Ichimura, Y. et al. (2000) Nature 408:488.

2. Ichimura, T. et al. (2004) J. Biol. Chem. 279:40584.

3. Yamaguchi, M. et al. (2010) J. Biol. Chem. 285:29599.

4. Otomo, C. et al. (2013) Nat. Struct. Mol. Biol. 20:59.

5. Shpilka, T. et al. (2012) Genome Biol. 12:226. 

• Long Name:

  Autophagy Related 3 Homolog

• Entrez Gene IDs:

  64422 (Human); 67841 (Mouse); 171415 (Rat)

• Alternate Names:

  APG3 autophagy 3-like (S. cerevisiae); APG3; APG3L; APG3-LIKE; Apg3p; ATG3 autophagy related 3 homolog (S. cerevisiae); ATG3; Autophagy-related protein 3; DKFZp564M1178; EC 6.3.2.-; FLJ22125,2610016C12Rik; hApg3; MGC15201; PC3-96; Protein PC3-96; ubiquitin-like-conjugating enzyme ATG3