• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells. When 5 x 10    ⁴ cells/well are added to mouse MAdCAM-1/Fc Chimera coated plates (10 µg/mL with 100 µL/well), approximately 80-90% will adhere after MnCl    ₂ stimulation for 1 hour incubation at room temperature.    
     Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse MAdCAM-1 (Gln22 - Thr365) Accession # NP_038619	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln22 predicted    
   

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  63 kDa (monomer)

• SDS-PAGE

  85-95 kDa, reducing conditions

Background: MAdCAM-1

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an immunoglobulin (Ig) cell adhesion molecule family member. In addition to Ig domains, it contains a mucin-like domain and a membrane proximal domain with similarity to IgA. MAdCAM-1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyer’s patches. It has also been found to be expressed on sinus-lining cells of the spleen. The integrin, alpha ₄ beta ₇, has been shown to function as the MAdCAM-1 receptor. The Ig domains of MAdCAM-1 have been found to be critical to alpha ₄ beta ₇ binding. The mucin domain has been shown to have activity in L-Selectin binding. MAdCAM-1 expression has been demonstrated to be up-regulated by TNF-alpha and IL-1 beta. MAdCAM-1 appears to play a role in inflammatory bowel disease (IBD) as its expression is highly up-regulated in IBD and most likely serves to recruit alpha ₄ beta ₇-expressing lymphocytes to the region. In vivo studies involving nonobese diabetic (NOD) mice have also suggested that MAdCAM-1/ alpha ₄ beta ₇ interaction plays a role in diabetes development in this model. Mouse MAdCAM-1 is a 405 amino acid (aa) residue protein with a 21 aa signal sequence, a 344 aa extracellular domain, a 20 aa transmembrane domain and a 20 aa cytoplasmic domain.

• References:

1. Briskin, M.J. et al. (1993) Nature 363:461.

2. Yang, X.D. et al. (1997) Diabetes 46:1542.

3. Sampaio, S.O. et al. (1995) J. Immunol. 155:2477.

4. Kraal, G. et al. (1995) Am. J. Pathol. 147:763.

5. Berg, E.L. et al. (1993) Nature 366:695.

6. Takeuchi, M. and V.R. Baichwal (1995) Proc. Natl. Acad. Sci. USA 92:3561.

• Long Name:

  Mucosal Addressin Cell Adhesion Molecule 1

• Entrez Gene IDs:

  8174 (Human); 17123 (Mouse)

• Alternate Names:

  hMAdCAM-1; MACAM1; MAdCAM1; MAdCAM-1; mucosal addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule-1; mucosal vascular addressin cell adhesion molecule 1