Human Pancreatic Polypeptide/PP MAb (Clone 548301) 100 UG

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Human Pancreatic Polypeptide/PP MAb (Clone 548301) 100 UG信息二维码

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产品介绍

    基本参数

    详细说明

    • Species Reactivity

      Human

    • Specificity

      Detects Pancreatic Polypeptide/PP in direct ELISAs and Western blots.

    • Source

      Monoclonal Mouse IgG    2B Clone # 548301

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      E. coli-derived recombinant human Pancreatic Polypeptide/PP    
      Ala30-Leu95    
      Accession # P01298

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      2 µg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human Pancreatic Polypeptide by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF Membrane was probed with 2 µg/mL of Human Pancreatic Polypeptide Monoclonal Antibody (Catalog # MAB6297) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for Pancreatic Polypeptide at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.2 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: Pancreatic Polypeptide/PP

    Pancreatic Polypeptide (PP) is an 11 kDa (calculated), unglycosylated member of the Neuropeptide-Y family of secreted peptide hormones. Human PP is synthesized with a 29 amino acid (aa) signal sequence and a 66 aa prohormone that contains the 36 aa PP hormone, a 20 aa icosapeptide of unknown function, and a C-terminal prosequence. PP is produced by pancreatic islet F-cells and released to the circulation following a meal. It slows stomach emptying time and insulin secretion and is thought to inhibit further food intake. The human PP prohormone shares 57% and 55% aa identity with mouse and rat PP, respectively.

    • Entrez Gene IDs:

      5539 (Human)

    • Alternate Names:

      INN; Obinepitide; pancreatic polypeptide (ppy); pancreatic polypeptide PNPPP; pancreatic polypeptide Y; Pancreatic Polypeptide; pancreatic prohormone; PNP; PP; PPY




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