• Species Reactivity

  Human

• Specificity

  Detects human TCF7L1 in direct ELISAs. In direct ELISAs, 5-10% cross-reactivity with recombinant human (rh) TCF-4 (aa 102-516) and rhTCF-12 is observed. No cross-reactivity with rhTCF7L1 (aa 151-320), rhTCF7L2, rhTCF-1, -2, -3, -4 (aa 241-365), rhLEF-1, or recombinant mouse TCF-1 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 622782

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E.coli-derived recombinant human TCF7L1    
  Lys429-Ser581    
  Accession # Q9HCS4

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF7L1/TCF3

Transcription factor 7-like 1 (TCF7L1; also HMG box transcription factor 3, TCF-3) is a 63 kDa member of the TCF/LEF family of proteins.  Human TCF7L1 is 588 amino acids (aa) in length and contains one HMG box DNA-binding domain (aa 346-414).  Human TCF7L1 shares 95% aa sequence identity with mouse TCF7L1.  TCF7L1 is expressed in hair follicles and skin keratinocytes and at lower levels in stomach epithelium.  Functionally, it participates in the Wnt signaling pathway.  TCF7L1 binds to DNA and acts as a repressor in the absence of CTNNB1 and as an activator in its presence.  TCF7L1 is also necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules, and the development of the barrier function of the epidermis.

• Long Name:

  Transcription Factor 7-like 1

• Entrez Gene IDs:

  83439 (Human); 21415 (Mouse); 312451 (Rat)

• Alternate Names:

  HMG box transcription factor 3; TCF3; TCF-3; TCF7L1; transcription factor 7-like 1 (T-cell specific, HMG-box); transcription factor 7-like 1