• Species Reactivity

  Human

• Specificity

  Detects human Kallikrein 5 Propeptide in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Kallikrein 5 (mature), rhKallikrein 1, -3, -4, -6, -7, -8, -9, -10, -11, -12, -13, -14, -15, rhKallikrein B1, rhHGFA, rhFactor VII, rhFactor X, rhFactor XI, rhThrombin, or rhuPA-1 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 325302

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Kallikrein 5    
  Val23-Arg66    
  Accession # NP_036559

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Kallikrein 5 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 5

Human tissue Kallikrein 5 (hK5, also known as stratum corneum tryptic enzyme) is a serine protease of the human tissue Kallikrein gene family (1, 2). The deduced amino acid sequence of human Kallikrein 5 consists of a signal peptide (Met1 to Gly22), a pro region (Val23 to Arg66) and a mature/active enzyme (Ile67 to Ser293). Kallikrein 5 is expressed mainly in skin, breast, brain and testis and to a lesser extent in many other tissues (3). Kallikrein 5 overexpression is an indicator of poor prognosis in ovarian cancer (4). Kallikrein 5 is a candidate physiological activator of Kallikrein 7 in the stratum corneum (5).

• References:

1. Brattsand, M. and T. Egelrud (1999) J. Biol. Chem. 274:30033.

2. Yousef, G.M. et al. (1999) Anticancer Res. 19:2843.

3. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.

4. Yousef, G.M. and E.P. Diamandis (1999) J. Biol. Chem. 274:37511.

5. Kim, H. et al. (2001) Br. J. Cancer 84:643.

• Entrez Gene IDs:

  25818 (Human); 68668 (Mouse); 690603 (Rat)

• Alternate Names:

  EC 3.4.21; EC 3.4.21.4; Kallikrein 5; Kallikrein-like protein 2; kallikrein-related peptidase 5; KLK5; KLKL2; KLK-L2; KLK-L2EC 3.4.21.-; SCTE; SCTEkallikrein-5; Stratum corneum tryptic enzyme