• Species Reactivity

  Human

• Specificity

  Detects human VE-Cadherin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Cadherin‑8, rhCadherin-17, rhE-Cadherin, rhN-Cadherin, rhP-Cadherin, or recombinant mouse VE-Cadherin is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 123413

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin    
  Asp48-Gln593    
  Accession # P33151

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

• Label

  Biotin

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human VE-Cadherin Fc Chimera (Catalog # )

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• Flow Cytometry

  2.5 µg/10    ⁶ cells

  HUVEC human umbilical vein endothelial cells stained in buffer containing Ca    ²⁺ and Mg    ²⁺  

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VE-Cadherin

Vascular endothelial (VE)-cadherin (VE-CAD), also called 7B4 and cadherin‑5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-cadherin proteins share approximately 74% homology.

• References:

1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.

2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.

3. Overduin, M. et al. (1995) Science 267:386.

4. Takeichi, M. (1991) Science 251:1451.

5. Nose, A. et al. (1987) EMBO J. 6:3655.

6. Carmeliet, P. et al. (1999) Cell 98:147.

7. Gory-Faure, S. et al. (1999) Development 126:2093.

• Long Name:

  Vascular Endothelium Cadherin

• Entrez Gene IDs:

  1003 (Human); 12562 (Mouse)

• Alternate Names:

  7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin