• Specificity

  Detects N-terminal and C-terminal V5 epitope-tagged proteins in Western blots.

• Source

  Recombinant Monoclonal Rabbit IgG Clone # 1036H

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  V5 epitope tag peptide    
  Accession # Y138513

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  See below

• 
  

• Simple Western

  1 µg/mL

  See below

• 
  

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

• Immunoprecipitation

  1-4 µg/100 µg cell lysate

  HEK293 human embryonic kidney cell line transfected with N-terminal V5-tagged FDNC4 and C-terminal V5-tagged EAA1, .

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: V5 Epitope Tag

• Entrez Gene IDs:

  3160800 (Viral)

• Alternate Names:

  GKPIPNPLLGLDST Epitope Tag; Parainfluenza virus 5 V/P; Paramyxovirus SV5; V5 Epitope Tag