• Species Reactivity

  Human

• Specificity

  Detects human Tryptase  epsilon /BSSP-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Tryptase epsilon /BSSP-4 is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 725445

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Tryptase  epsilon /BSSP-4    
  Ala33-Ser317    
  Accession # Q9GZN4

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase epsilon/BSSP-4

Tryptase epsilon, also known as brain-specific protease-4 (BSSP-4) and prosemin, is expressed in the brain and epithelium-rich tissues such as the lung and eye (1, 2). BSSP-4 is a serine protease related to trypsin, preferentially hydrolyzing substrates after arginine and lysine residues. However, Tryptase epsilon is less susceptible to inhibition by common trypsin inhibitors such as aprotinin, alpha 1-antitrypsin and secretory leukocyte protease inhibitor (3). Tryptase  epsilon efficiently converts pro-urokinase-type plasminogen activator to its mature, active form (3).

• References:

1. Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.

2. Wong, G.W. et al. (2001) J. Biol. Chem. 276:49169.

3. Yasuda, S. et al. (2005) Blood 105:3893.

• Entrez Gene IDs:

  64063 (Human); 70835 (Mouse)

• Alternate Names:

  BSP-2; BSSP4; BSSP-4; hBSSP-4; MGC9599; prosemin; protease, serine S1 family member 22; protease, serine, 22; PRSS22; serine protease 22; serine protease 26; SP001LA; Tryptase epsilon; UNQ302/PRO343