详细说明
Species Reactivity
Human
Specificity
Detects human M-CSF in flow cytometry.
Source
Monoclonal Mouse IgG 2A Clone # 26786
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human M-CSF
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry | Detection of M‑CSF in Human Macrophages by Flow Cytometry. Human macrophages treated with LPS and Recombinant Human TNF-alpha (Catalog # ) were stained with Mouse Anti-Human M‑CSF PE‑conjugated Monoclonal Antibody (Catalog # IC2161P, filled histogram) or isotype control antibody (Catalog # , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: M-CSF
M-CSF, also known as CSF-1, is produced by a variety of cell types in either a membrane-anchored or secreted soluble form. It is a glycosylated, disulfide-linked homodimer that binds to the M-CSF receptor expressed by cells of the monocyte-macrophage lineage.
Long Name:
Macrophage Colony Stimulating Factor
Entrez Gene IDs:
1435 (Human); 12977 (Mouse)
Alternate Names:
colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930