• Species Reactivity

  Human

• Specificity

  Detects human CD45. This antibody recognizes all isoforms of human CD45.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 2D1

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human peripheral blood mononuclear cells

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

• Label

  Biotin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  Human peripheral blood mononuclear cells

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD45

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor‑like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The CD45RABC cDNA encodes 1304 amino acids (aa), including a 23 aa signal sequence, a 552 aa extracellular domain containing the splicing region, a cysteine‑rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 707 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 32‑191. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine‑tuned by varying usage of the heavily
O-glycosylated spliced regions and sialylation of N‑linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP‑1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

• References:

1. Anderson, J.N. et al. (2004) FASEB J. 18:8.

2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.

3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.

4. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.

5. Ralph, S.J. et al. (1987) EMBO J. 6:1251.

6. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.

7. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.

8. McNiell, L. et al. (2007) Immunity 27:425.

9. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.

10. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.

11. Zhu, J.W. et al. (2008) Immunity 28:183.

12. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.

13. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.

14. Cross, J.L. et al. (2008) J. Immunol. 180:8020.

• Long Name:

  Cluster of Differentiation 45

• Entrez Gene IDs:

  5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)

• Alternate Names:

  B220; CD45 antigen; CD45; CD45R; EC 3.1.3.48; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide