• Species Reactivity

  Human, Mouse

• Specificity

  Detects human RND3 in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 687202

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  RND3 (RD1 peptide)    
  Cys197-Arg207    
  Accession # P61587

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RND3

RND3, also known as RhoE, is an approximately 27 kDa member of the Rho family, small GTPase superfamily of molecules.  It is 244 amino acids (aa) in length, and the mature chain extends from aa 1-241.  RND3 is ubiquitously expressed and is involved in keratinocyte differentiation and stratification.  RND3 overexpression results in cell size enlargement and an increase in the number of stratified cells.  RND3 depletion results in hyperproliferation and delays initiation of keratinocyte differentiation.  Human RND3 shares 100% aa sequence identity with mouse RND3.

• Long Name:

  Rho family GTPase 3

• Entrez Gene IDs:

  390 (Human)

• Alternate Names:

  ARHE; MemB; Protein MemB; Rho family GTPase 3member E; Rho8; RHOE; Rho-related GTP-binding protein Rho8; rho-related GTP-binding protein RhoE; RND3; small GTP binding protein Rho8