• Species Reactivity

  Human

• Specificity

  Detects human IL‑36 gamma /IL‑1F9 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-36Ra/IL-1F5, rhIL-36 alpha /IL-1F6, rhIL-1F7, rhIL-36 beta /IL-1F8, rhIL-1ra/IL-1F3, or rhIL-18/IL-1F4 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 278706

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human IL‑36 gamma /IL‑1F9    
  Met1-Asp169    
  Accession # Q9NZH8

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human IL‑36 gamma /IL‑1F9 (Catalog # )

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• Blockade of Receptor-ligand Interaction

  In a functional ELISA, 0.15-0.6 µg/mL of this antibody will block 50% of the binding of 5 µg/mL of Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera to immobilized Recombinant Human IL‑36 gamma /IL‑1F9 coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding.

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 gamma/IL-1F9

Interleukin-36 gamma (IL-36 gamma ) [also named IL-1F9, IL-1 epsilon  (epsilon), and IL-1H1] is a member of the IL-1 family, which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (1‑4). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-36 gamma is synthesized as a 19 kDa, 169 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site (1, 2, 5). The molecule is secreted when transfected into 293T cells (5). Human to mouse, IL-36 gamma has 53% aa sequence identity. Within the family, IL-36 gamma shares 30%, 23%, 33%, 57%, 35%, 45%, and 32% aa sequence identity with IL-1 ra, IL-1 beta, IL-1F5, F6, F7, F8, and F10, respectively. Cells reported to express IL-36 gamma include Langerhans cells, keratinocytes/stratified squamous epithelium, plus chief cells and parietal cells of the stomach (4, 5). The receptor for IL-36 gamma is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (6). Recombinant IL-36 gamma, along with IL-36 beta /IL-1F8 and IL-36 alpha /IL-1F6, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.

• References:

1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.

2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.

3. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.

4. Dunn, E. et al. (2001) Trends Immunol. 22:533.

5. Debets, R. et al. (2001) J. Immunol. 167:1440.

6. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

• Long Name:

  Interleukin 36 gamma/Interleukin 1 Family 9

• Entrez Gene IDs:

  56300 (Human); 215257 (Mouse); 499744 (Rat)

• Alternate Names:

  IL-1 epsilon; IL-1 H1; IL-1 Related Protein 2; IL-1(EPSILON); IL1E; IL-1-epsilon; IL1F9; IL-1F9; IL1H1; IL-1H1; IL-1-Related Protein 2; IL1RP2; IL-1rp2; IL36 gamma; IL-36 gamma; IL36G; interleukin 1 family, member 9; interleukin 1-related protein 2; Interleukin 36, Gamma; Interleukin-1 epsilon; interleukin-1 family member 9; Interleukin-1 homolog 1; Interleukin-36 Gamma