详细说明
Species Reactivity
Human
Specificity
Detects human CXCL5/ENA-78 in ELISAs. Does not cross-react with recombinant human CXCL1, 2, or 3.
Source
Monoclonal Mouse IgG 1 Clone # 33170
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CXCL5/ENA-78
Ala37-Asn114
Accession # P42830Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/10 6 cells
See below
Human CXCL5/ENA-78 Sandwich Immunoassay
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL
Human CXCL5/ENA‑78 Antibody (Catalog # )
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL
Human CXCL5/ENA‑78 Biotinylated Antibody (Catalog # )
ELISA Standard
Recombinant Human CXCL5/ENA-78 Protein (Catalog # )
Neutralization
Measured by its ability to neutralize CXCL5/ENA‑78-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND 50) is typically 3-12 µg/mL in the presence of 30 ng/mL Recombinant Human CXCL5/ENA‑78.
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry | Detection of CXCL5/ENA‑78 in Human PBMCs by Flow Cytometry. Human peripheral blood mononculear cells (PBMCs) either treated with LPS (filled histogram) or untreated (open histogram) were stained with Mouse Anti-Human CXCL5/ENA‑78 Monoclonal Antibody (Catalog # MAB654), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
Neutralization | Chemotaxis Induced by CXCL5/ENA‑78 and Neutralization by Human CXCL5/ENA‑78 Antibody. Recombinant Human CXCL5/ENA‑78 (Catalog # ) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # ). Chemotaxis elicited by Recombinant Human CXCL5/ ENA‑78 (30 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL5/ENA‑78 Monoclonal Antibody (Catalog # MAB654). The ND50 is typically 3-12 µg/mL. |
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL5/ENA-78
CXCL5, also known as ENA-78, is a CXC chemokine that plays a role in inflammatory responses and angiogenesis.
Entrez Gene IDs:
6374 (Human)
Alternate Names:
CXCL5; CXCL5/ENA-78; ENA78; ENA-78
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