• Species Reactivity

  Human

• Specificity

  Detects human Cystatin C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cystatins A, B, D, E/M, S, SA, SN, rhFetuins A, B, or rhHPRG (Histidine-Proline-Rich Glycoprotein) is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 197807

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Cystatin C    
  Ser27-Ala146    
  Accession # P01034

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1-2 µg/mL

  See below

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Human Cystatin C (Catalog # ),

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin C

Cystatin C is a member of family 2 of the Cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). It is ubiquitous in human tissues and body fluids. A point mutation in the gene coding for the 120 amino acid mature Cystatin C causes a hereditary form of amyloid angiopathy in which the protein variant (Leu68 to Gln) is deposited in the cerebral arteries, leading to fatal cerebral hemorrhage (4). Cystatin C may have additional clinical applications. For example, it is a good marker for glomerular filtration rate (5).

• References:

1. Reed, C.H. (2000) British J. Biomed. Sci. 57:323.

2. Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.

3. Abrahamson, M. (1994) Methods Enzymol. 244:685.

4. Abrahamson, M. et al. (1992) Hum. Genet. 89:377.

5. Laterza, O.F. et al. (2002) Clin. Chem. 48:699.

• Entrez Gene IDs:

  1471 (Human); 13010 (Mouse); 25307 (Rat)

• Alternate Names:

  ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin