详细说明
Species Reactivity
Mouse
Specificity
Detects mouse Rae‑1 epsilon in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Rae-1 alpha, beta, gamma, or δ is observed. By flow cytometry, no cross-reactivity with mouse Rae-1 alpha or mouse Rae-1 gamma.
Source
Monoclonal Rat IgG 2A Clone # 205001
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with mouse Rae-1 epsilon
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Flow Cytometry
2.5 µg/10 6 cells
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.3-0.9 µg/mL of this antibody will block 50% of the binding of 125 ng/mL of Recombinant Mouse NKG2D Fc Chimera (Catalog # ) to immobilized Recombinant Mouse Rae-1 epsilon Fc Chimera (Catalog # ) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of Rae‑1 epsilon in BaF3 Mouse Cell Line Transfected with Mouse Rae-1 epsilon and eGFP by Flow Cytometry. BaF3 mouse pro-B cell line transfected with (A) mouse Rae-1 epsilon or (B) mouse Rae-1 alpha and eGFP was stained with Rat Anti-Mouse Rae‑1 epsilon Monoclonal Antibody (Catalog # MAB1135) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # ). Quadrant markers were set based on control antibody staining (Catalog # ). |
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rae-1 epsilon
Rae-1 epsilon is a member of a family of cell-surface proteins that function as ligands for mouse NKG2D. Other family members are designated Rae-1 alpha, beta, gamma, and δ. Amino acid sequence identity within this family ranges from 88‑95%. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig‑like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. The Rae-1 proteins are anchored to the membrane via a GPI‑linkage. The name of this family derives from the original identification of these proteins as the product of retinoic acid early inducible transcripts. Rae-1 expression is developmentally controlled. Transcripts were observed in the brain/head region of day 10‑14 embryos but disappeared by day 18. Rae-1 transcripts were detected in several transformed cell lines but are absent from most normal adult tissues. All Rae-1 family members bind to mouse NKG2D, an activating receptor expressed on NK cells and some T cell subsets, resulting in the activation of cytolytic activity and/or cytokine production by these effector cells. Ectopic expression of Rae-1 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1‑7).
References:
Zou, Z. et al. (1996) J. Biochem (Tokyo) 119:319.
Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
Cerwenka, A. et al. (2000) Immunity 12:721.
Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
Diefenbach, A. et al. (2001) Nature 413:165.
Champsaur, M. et al. (2010) J. Immunol. 185:157.
Markiewicz M. et al. (2012) Immunity 36:132.
Long Name:
Retinoic Acid Early Transcript 1 epsilon
Entrez Gene IDs:
8480 (Human); 379043 (Mouse)
Alternate Names:
Rae1 epsilon; Rae-1 epsilon