• Assay Type

  Solid Phase Sandwich ELISA

• Format

  96-well strip plate

• Range

  62.50 - 4,000 pg/mL

• Sufficient Materials

  For five 96-well plates*

• Specificity

  Please see the

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Ancillary Reagent Kit Available  

DY008, DuoSet ELISA Ancillary Reagent Kit 2 -

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Fatty Acid-Binding Protein 4 (FABP4). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and palsma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

• Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time

• Development protocols are provided to guide further assay optimization

• Assay can be customized to your specific needs

• Economical alternative to complete kits

Kit Content

• Capture Antibody

• Detection Antibody

• Recombinant Standard

• Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # ) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.  
 
  The components listed above may be purchased separately:  
  PBS: (Catalog # ), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na  ₂HPO  ₄, 1.5 mM KH  ₂PO  ₄, pH 7.2 - 7.4, 0.2 µm filtered  
 
  Wash Buffer: (Catalog # ), or 0.05% Tween  ^® 20 in PBS, pH 7.2-7.4  
  Reagent Diluent: (Catalog # ), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered  
 
  Substrate Solution: 1:1 mixture of Color Reagent A (H  ₂O  ₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # )  
 
  Stop Solution: 2 N H  ₂SO  ₄ (Catalog # )  
  Microplates: R&D Systems (Catalog # )  
 
  Plate Sealers: ELISA Plate Sealers (Catalog # )

Preparation and Storage

• Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FABP4/A-FABP

FABP4 (Fatty Acid Binding Protein 4), also known as A-FABP and aP2, is the predominant FABP found in adipocytes and is often used as a marker for adipocyte differentiation. It is also expressed in macrophages, dendritic cells, and endothelial cells. FABP4 is a key mediator of intracellular fatty acid transport and metabolism in adipose tissue. Its expression is regulated by multiple factors including fatty acids, PPAR gamma agonists, and Insulin, and its levels increase with lipolytic stimulation. It can increase the hydrolytic activity of Hormone-Sensitive Lipase and increase the production of proinflammatory cytokines and chemokines. FABP4 upregulation in adipocytes and macrophages is associated with the development of Insulin resistance, hypertriacylglycerolaemia, and atherosclerosis. Serum levels of FABP4 are elevated in obesity and metabolic syndrome, type 2 diabetes, HIV-associated lipodystrophy, polycystic ovary syndrome, nonalcoholic fatty liver disease, atherosclerosis, and acute ischaemic stroke. FABP4 is also overexpressed in multiple cancer types including ovarian, bladder, glioblastoma, and oral, and it may play a role in tumor progression.

• Entrez Gene IDs:

  2167 (Human); 11770 (Mouse); 79451 (Rat);

• Long Name:

  Fatty Acid-Binding Protein 4

• Aliases:

  Adipocyte lipid-binding protein; Adipocyte-type fatty acid-binding protein; AFABP; A-FABP; A-FABPAFABP; ALBP; aP2; FABP4; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; fatty acid-binding protein, adipocyte