• Assay Type

  Solid Phase Sandwich ELISA

• Format

  96-well strip plate

• Range

  15.60 - 1,000 pg/mL

• Sufficient Materials

  For five 96-well plates*

• Specificity

  Please see the

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Ancillary Reagent Kit Available  

DY008, DuoSet ELISA Ancillary Reagent Kit 2 -

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Meteorin-like protein (METRNL). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

• Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time

• Development protocols are provided to guide further assay optimization

• Assay can be customized to your specific needs

• Economical alternative to complete kits

Kit Content

• Capture Antibody

• Detection Antibody

• Recombinant Standard

• Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # ) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # ), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # ), or 0.05% Tween^® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # ), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # )

Stop Solution: 2 N H₂SO₄ (Catalog # )

Microplates: R&D Systems (Catalog # )

Plate Sealers: ELISA Plate Sealers (Catalog # )

Preparation and Storage

• Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Meteorin-like/METRNL

METRNL (Meteorin-like) is a cytoplasmic 30 kDa (predicted) member of the meteorin family of proteins. The name meteorin derives from the ability of the founding family member meteorin to transform glia into cells containing an extensive, meteor-like tail. It is expressed by neurons, and appears to function as a primer molecule that sets the stage for either cell differentiation or neurite outgrowth when it acts with Dclk1 and SerpinB1. Mature human METRNL is 266 amino acids (aa) in length (aa 46-311). It reportedly contains a signal sequence (aa 1-45), which suggests it may be secreted. Mature human and mouse METRNL share 79% aa sequence identity.

• Entrez Gene IDs:

  284207 (Human); 210029 (Mouse); 316842 (Rat);

• Aliases:

  Meteorin like; meteorin, glial cell differentiation regulator-like; meteorin-like protein; Meteorin-like; METRNL; MGC99788